Metal Content and Biochemical Analyses of a Recombinant Collagenase PrtV fromVibrio parahaemolyticus
نویسندگان
چکیده
منابع مشابه
Expression and characterization of the prtV gene encoding a collagenase from Vibrio parahaemolyticus in Escherichia coli.
The prtV gene, encoding a collagenase of Vibrio parahaemolyticus, was expressed in Escherichia coli and purified by affinity chromatography. The transformant E. coli BL21(DE3)(pPRT2) secreted the recombinant PrtV, and the highest enzyme activity was detected in the culture supernatant after 5 h IPTG induction. The molecular mass of purified PrtV was 62 kDa as determined by gel filtration, which...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2000
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.2000.tb02567.x